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KMID : 0357920020360040212
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Korean Journal of Pathology
2002 Volume.36 No. 4 p.212 ~ p.221
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Prenatal Development of Human Lip with Immunohistochemical Study
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Hong Su-Jung
Lee Young-Joon
Kim Yeon-Sook
Lee Suk-Keun
Chi Je-Geun
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Abstract
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Background: This study is aimed to elucidate the developmental pattern of human fetal lip by histological and immunohistochemical examinations.
Methods: Totally 231 normal human lip tissues obtained from autopsied fetuses were fixed with 10% buffered formalin, sectioned in cross and longitudinal directions, routinely stained for H&E and performed for immunohistochemistry with antibodies of S-100 protein, proliferating cell nuclear antigen (PCNA), transglutaminase C (TGase-C), metalloproteinase (MMP)-3, MMP-10, tenascin, KL1, K8.12, E-cadherin, tissue inhibitors of matrix metalloproteinase (TIMP)-1, TIMP-2 and total keratin (TK).
Results: The lip structure first appeared as an orifice of stomodeum around the 7-8th week of gestation, and a major structure of the midface was observed by the 11-12th week. As the squamous epithelium of the lip became thick and was keratinized, the vermilion border became distinguished in the 15-16th week, and the lip structure was almost completed with the presence of orbicularis oris muscle in the lingual side of vermilion border by the 17-18th week. Immunohistochemically, the vermilion border showed strong reactions for tenascin, E-cadherin and MMP-3 and increased positivity for PCNA, cytokeratins (TK, KL1, K8.12), and TGase-C.
Conclusion: With the above findings we suppose that the cytodifferentiation of vermilion border epithelium plays an important role for the development of human fetal lip.
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KEYWORD
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Fetal Development, Lip, Immunohistochemistry
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